
Thermo Fisher Scientific Goat anti-Human IgG Fc Highly Cross-Adsorbed Secondary Antibody, HRP
Thermo Fisher의 염소 유래 anti-Human IgG Fc HRP 결합 2차 항체로, Western blot, IHC, ELISA에 적합합니다. 고도로 교차 흡착되어 비특이적 결합을 최소화하며, 높은 민감도와 신뢰성을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobin Fc region |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535606 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, horse, mouse, and rabbit serum proteins, as well as human IgA and IgM |
| Antibody Form | Whole Antibody |
Additional Formats Available
- AP conjugate
- Biotin conjugate
- FITC conjugate
- TRITC conjugate
- Custom conjugation available upon request
Product Specific Information
Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8°C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum proteins, light chains on all human immunoglobulins, human IgA or IgM, or serum proteins from bovine, mouse, or rabbit.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins.
They are useful in detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility, enabling the use of multiple detection systems (e.g., HRP, AP, fluorescence).
They also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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