Thermo Fisher Scientific Actin Recombinant Rabbit Monoclonal Antibody (JJ09-29)
Actin 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC에 적합합니다. 높은 특이성과 재현성을 제공하며, 로트 간 일관성이 우수합니다. 단백질 A 정제, 1 mg/mL 농도, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000 | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JJ09-29 |
| Immunogen | Synthetic peptide within Human Actin (aa 45–80) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809756 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal production candidates.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
Actin is a ubiquitous protein involved in filament formation, constituting a major part of the cytoskeleton. Actin filaments interact with myosin to facilitate muscle contraction, cell motility, and cytokinesis.
In vertebrates, actin isoforms are categorized into three groups: alpha, beta, and gamma.
- Alpha actins: Found in muscle tissues; key component of the contractile apparatus
- Beta and gamma actins: Present in most cell types; contribute to cytoskeletal structure and internal cell motility
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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