Thermo Fisher Scientific SUMO4 Recombinant Rabbit Monoclonal Antibody (JJ085-01)
SUMO4 단백질을 인식하는 재조합 토끼 단클론 항체로 높은 특이성과 재현성을 제공. Western blot, ICC/IF, Flow cytometry 등 다양한 응용 가능. HEK293 발현 시스템 기반으로 생산되어 동물 유래 성분이 없음. 단기 4°C, 장기 -20°C 보관 권장.
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Thermo Fisher Scientific SUMO4 Recombinant Rabbit Monoclonal Antibody (JJ085-01)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JJ085-01 |
| Immunogen | Recombinant protein within Human SUMO4 aa 1–105 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809755 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to larger rabbit immune repertoire
Target Information
SUMO4 is a member of the SUMO gene family, encoding small ubiquitin-related modifiers that covalently attach to target lysines, influencing protein localization, stability, and activity.
Under oxidative stress, SUMO4 conjugates to antioxidant enzymes and stress defense proteins, modulating transcriptional activity of NFKBIA, TFAP2A, FOS, and NR3C1.
Unlike SUMO1–3, SUMO4 is resistant to sentrin-specific proteases due to Pro-90, affecting its maturation and conjugation.
SUMO4 localizes in the cytoplasm and negatively regulates NF-κB-dependent transcription of IL12B. The M55V substitution is associated with type I diabetes.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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