Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), Brilliant Ultra Violet 737, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 647, 700
• APC, Biotin
• Brilliant Violet 480, 711, 786
• Functional Grade
• NovaFluor Blue 660-120S, Red 700, Red 725, Red 755
• NovaFluor Yellow 610, 690, 700, 755
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436, 600, 645
• Request custom conjugation available

Product Specific Information

The IT2.2 monoclonal antibody reacts with human CD86 (~80 kDa), also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated by stimuli such as BCR complex, CD40, and cytokine receptors.

CD86 interacts with T-cell molecules CD28 and CD152 (CTLA-4), playing a key role in T-B cell crosstalk, costimulation, and immune regulation. The IT2.2 antibody is validated for flow cytometric analysis and pre-diluted for use at 5 µL (0.125 µg) per test.

Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is compatible with cytometers equipped with a 355 nm UV laser. Use staining buffers such as Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to minimize polymer dye interactions.

Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for short-term storage (up to 3 days at 4°C in dark).
Methanol Compatibility: Suitable for short-term methanol fixation (<1 hour).
Excitation: 355 nm; Emission: 732 nm; Laser: Ultraviolet.

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD86 is a ligand for CTLA4 and CD28, acting as a costimulatory molecule for T-cell activation. Insufficient co-stimulation can induce tolerance. CD86 is expressed by antigen-presenting cells and binds CD28 (activating T-cells) and CTLA-4 (inhibiting T-cell activation). Alternative splicing generates multiple isoforms. CD86 dysfunction is associated with gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures.

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