Thermo Fisher Scientific MCP-1 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL
• Recommended for Sandwich ELISA with PeproTech Anti-Rat MCP-1 (500-P76)

Product Specifications

Product Specific Information

AA Sequence of Recombinant Protein:
QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat MCP-1 (CCL2). Anti-Rat MCP-1 (CCL2)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Rat MCP-1 (MCAF) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat MCP-1 (MCAF) (500-P76) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Rat MCP-1 (MCAF).

Western Blot:
To detect Rat MCP-1 (MCAF) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Rat MCP-1 (MCAF) is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P76BT-1MG is provided as 2 × 500 µg.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are secreted proteins involved in immunoregulatory and inflammatory processes. The encoded protein belongs to the CXC cytokine subfamily, characterized by two cysteines separated by a single amino acid. MCP-1 displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It is implicated in diseases with monocytic infiltrates such as psoriasis, rheumatoid arthritis, and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.