
Thermo Fisher Scientific MCP-1 Polyclonal Antibody, PeproTech
Rat MCP-1(CCL2)에 특이적인 Rabbit Polyclonal Antibody로, Western Blot, ELISA, Immunohistochemistry 등 다양한 분석에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, Lyophilized 형태로 제공. 연구용으로만 사용.
- 카탈로그번호
- 500-P76-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific MCP-1 Polyclonal Antibody, PeproTech
Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| Immunohistochemistry (IHC) | - | 3 publications |
| ELISA | 0.5–2.0 µg/mL | 4 publications |
| Neutralization (Neu) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
| Immunostaining (IS) | - | 5 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Published Species | Pig, Rat |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Rat MCP-1 (CCL2) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930749 |
Product Specific Information
AA Sequence of recombinant protein:
QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat MCP-1 (CCL2). Anti-Rat MCP-1 (CCL2)-specific antibody was purified by affinity chromatography employing an immobilized Rat MCP-1 (CCL2) matrix.
Sandwich ELISA:
To detect Rat MCP-1 (CCL2) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. In conjunction with PeproTech Biotinylated Anti-Rat MCP-1 (CCL2) (500-P76Bt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Rat MCP-1 (CCL2).
Western Blot:
To detect Rat MCP-1 (MCAF) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Rat MCP-1 (MCAF) is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are secreted proteins involved in immunoregulatory and inflammatory processes. The encoded protein is structurally related to the CXC subfamily of cytokines, characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It is implicated in diseases with monocytic infiltrates such as psoriasis, rheumatoid arthritis, and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 500-P76-100UG_CCL2_MCP-1_P14844-1_Rabbit.svg, 500-P76-100UG_CCL2_MCP-1_P14844-1_Rabbit_PDP.jpeg)
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