Thermo Fisher Scientific CCL3 Polyclonal Antibody, Biotin, PeproTech
Rat MIP-1alpha(CCL3)을 인식하는 Biotin 결합 폴리클로날 항체. Western blot과 ELISA에 최적화되어 있으며, 항원 친화 크로마토그래피로 정제됨. PBS 버퍼에 동결건조 형태로 제공되며, -20°C 보관. 연구용 전용 제품.
- 카탈로그번호
- 500-P77BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CCL3 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.25–1.0 µg/mL | View 4 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Rat MIP-1alpha (CCL3) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930759 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
APYGADTPTA CCFSYGRQIP RKFIADYFET SSLCSQPGVI FLTKRNRQIC ADPKETWVQE YITELELNA
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat MIP-1alpha (CCL3). The antibody was purified by affinity chromatography and subsequently biotinylated.
Sandwich ELISA:
- Recommended concentration: 0.25–1.0 µg/mL (100 µL/well)
- Use with PeproTech Polyclonal Anti-Rat MIP-1alpha (500-P77) as capture antibody
- Detects ≥0.2–0.4 ng/well of recombinant Rat MIP-1alpha
Western Blot:
- Recommended concentration: 0.1–0.2 µg/mL
- Detection limit: 1.5–3.0 ng/lane under reducing or non-reducing conditions
- Use with compatible secondary reagents
Packaging:
500-P77BT-1MG is provided as 2 × 500 µg vials.
Target Information
MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines involved in host defense against bacterial, viral, parasitic, and fungal infections. They regulate trafficking and activation of inflammatory cells such as macrophages, lymphocytes, and NK cells.
- MIP-1alpha selectively attracts CD8+ lymphocytes
- MIP-1beta selectively attracts CD4+ lymphocytes
Both chemokines are potent chemoattractants for B cells, eosinophils, and dendritic cells, and are active on human and murine hematopoietic cells.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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