Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), APC, eBioscience
인간 TNF alpha를 인식하는 중화형 단클론 항체로, APC 형광 표지되어 유세포분석(Flow Cytometry)에 적합합니다. 5 µL/test로 사용되며, 면역세포 내 TNFα 검출에 활용됩니다. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
- Application: Flow Cytometry (Intracellular)
- Tested Dilution: 5 µL (0.125 µg)/test
- Publications: View 41 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MAb11 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1548821 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 660
- Alexa Fluor 700
- Biotin
- Brilliant UV 661 / 737
- Brilliant Violet 421 / 650
- eFluor 450
- FITC
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
Description:
The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNFα), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4⁺ T cells. TNFα exhibits cytolytic activity against various tumor cells and plays a key role in immune regulation. It forms dimers and trimers and also exists as a 26 kDa membrane-bound form. The MAb11 antibody is a neutralizing antibody.
Applications Reported:
Reported for use in intracellular flow cytometric analysis.
Applications Tested:
Tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00) and the BestProtocols® Protocol A.
- Pre-titrated for intracellular staining
- Use 5 µL (0.125 µg) per test (final volume 100 µL)
- Recommended cell number: 10⁵–10⁸ cells/test
- Excitation: 633–647 nm; Emission: 660 nm; Laser: Red
- 0.2 µm post-manufacturing filtered
Target Information
TNF alpha (Tumor Necrosis Factor-alpha):
A multifunctional proinflammatory cytokine belonging to the TNF superfamily. Secreted mainly by macrophages, it binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNFα regulates immune responses, cell proliferation, differentiation, apoptosis, and metabolism. It exists as multimeric forms (dimers, trimers, pentamers) but appears as a single 17 kDa band under non-reducing SDS-PAGE.
TNFα exhibits tumoricidal activity, synergizes with interferon-gamma, and is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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