Thermo Fisher Scientific FITC Monoclonal Antibody (NAWESLEE), APC, eBioscience

Applications

Immunocytochemistry (ICC/IF)

• Not tested
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Flow Cytometry (Flow)

• Tested Dilution: 0.125 µg/test
  View 1 publication

Miscellaneous PubMed (Misc)

• Not tested
  View 1 publication

Product Specifications

Product Specific Information

This NAWESLEE monoclonal antibody reacts to fluorescein isothiocyanate (FITC), a derivative of fluorescein commonly used in flow cytometry and fluorescent microscopy.
FITC-9 can be used for the separation of cells labeled with FITC-conjugated antibodies or for staining.
Please note that in some instances, the FITC signal can be quenched by the anti-FITC antibody; therefore, this phenomenon should be evaluated empirically.

Applications Reported:
This NAWESLEE antibody has been reported for use in flow cytometric analysis.

Applications Tested:
This NAWESLEE antibody has been tested by flow cytometric analysis of cells stained with FITC-conjugated antibody.
Recommended usage: ≤ 0.125 µg per test.
Higher concentrations may quench FITC fluorescence.
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.
Typical cell number per test: 10⁵–10⁸ cells.
Careful titration is recommended for optimal performance.

Optical Properties:
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light, causing molecules to emit visible yellow-green light.
Fluorochrome labeling enables rapid, accurate localization of antigen-antibody interactions.
FITC conjugation is simple and preserves protein activity, making it widely used in immunofluorescent applications.
Anti-FITC antibodies can be used for isolating FITC-labeled cells via magnetic sorting or reducing background signal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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