Thermo Fisher Scientific NFATC1 Monoclonal Antibody (7A6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

View 44 publications 44 publications

Immunohistochemistry (IHC)

-

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:250

View 12 publications 12 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

ChIP assay (ChIP)

Assay-dependent

View 8 publications 8 publications

Gel Shift (GS)

Assay-dependent

View 12 publications 12 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Hamster, Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7A6

Immunogen

Bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NFATC1,uniProtId:O95644-1,ncbiNodeId:9606,antigenRange:1-654,antigenLength:943,antigenImageFileName:MA3-024_NFATC1_O95644-1_House_mouse.svg,antigenImageFileNamePDP:MA3-024_NFATC1_O95644-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2236037

Product Specific Information

MA3-024 detects NFATc1 (NFAT2) from transfected human, rat, mouse, and monkey cells. This antibody does not cross react with NFATc2 (NFAT1).

MA3-024 has been successfully used in Western blot, immunoprecipitation, ChIP, immunofluorescence and gel shift procedures. By Western blot, this antibody detects an ~105 kDa band representing transfected NFAT2 from COS cell lysate.

The MA3-024 immunogen is bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654.

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell-based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization, including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody, may have to be performed for your high content assay.

Target Information

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.