Thermo Fisher Scientific TCP1 Monoclonal Antibody (91A)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

-

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

1/100

-

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Chicken, Hamster, Human, Mouse, Non-human primate, Rat, Yeast

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

91A

Immunogen

Bacterially expressed fragment of mouse TCP-1, residues 306-556. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TCP1,uniProtId:P11983-1,ncbiNodeId:10090,antigenRange:306-556,antigenLength:556,antigenImageFileName:MA3-026_TCP1_P11983-1_Rat.svg,antigenImageFileNamePDP:MA3-026_TCP1_P11983-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2303275

Product Specific Information

MA3-026 detects T Complex Polypeptide 1 (TCP-1) from human, canine, chicken, hamster, mouse, primate, rat and yeast samples.

MA3-026 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this clone detects a ~57 kDa band representing TCP-1 in mouse 3T3 cells. Immunofluorescence staining of TCP-1 in mouse germ line cells with MA3-026 results in diffuse cytoplasmic staining.

The MA3-026 immunizing peptide corresponds to amino acid residues 306-556 from mouse TCP-1. The epitope for this antibody has been mapped to amino acids 465-469 representing the pentamer peptide AKLRA.

Target Information

The T Complex Polypeptide 1 (TCP-1) is approximately 60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP-1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.