Thermo Fisher Scientific HSP60 Monoclonal Antibody (A57-B9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

-

View 8 publications 8 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bacteria

Published species

Bacteria, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A57-B9

Immunogen

Recombinant Chlamydia trachomatis HSP60 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325470

Product Specific Information

MA3-023 detects heat shock protein 60 kDa (HSP60) from bacteria. This antibody detects all three species of Chlamydia HSP60 with minimal cross-reactivity with Borrelia burgdorferi HSP60. MA3-023 does not react with E. coli GroEL.

MA3-023 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a single ~60 kDa band representing HSP60 from C. trachomatis lysate.

The MA3-023 immunogen is recombinant Chlamydia trachomatis HSP60. Epitope mapping studies suggest that this antibody binds to a linear sequence of Chlamydia HSP60 amino acids 517-522.

Target Information

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.