Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), FITC, eBioscience
Ki-67 단백질을 인식하는 20Raj1 클론의 FITC 결합 단일클론 항체로, 세포 증식 마커 분석에 적합합니다. 인간 및 개 시료에서 반응하며, Flow Cytometry, IHC, ICC/IF 등 다양한 응용에 사용 가능합니다. 488 nm 청색 레이저에 최적화되어 있습니다.
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Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | View 2 publications |
| Flow Cytometry (Flow) | 0.06 µg/test | View 10 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Published Species | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 20Raj1 |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11039527 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450 / 506 / 570 / 615 / 660
- PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 (345 and 395 kDa) are expressed in dividing cells and absent in resting cells (G0). During interphase, Ki-67 localizes to the nucleus, redistributing to chromosomes during mitosis, and associates with heterochromatin-bound proteins such as CBX3.
In tumor studies, Ki-67 expression serves as a marker for the fraction of proliferating cells.
This antibody also recognizes canine Ki-67.
Applications Reported: Intracellular staining followed by flow cytometric analysis, immunohistochemistry, and immunocytochemistry.
Applications Tested: Pre-titrated and tested by intracellular staining and flow cytometric analysis using Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00).
Use at 5 µL (0.06 µg) per test in a final volume of 100 µL. Suitable for 10⁵–10⁸ cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus through its FHA domain.
Ki-67 expression varies by cell cycle stage: perinucleolar in G1, nuclear matrix in G2, and chromosomal during M phase.
It serves as a proliferation marker, undetectable in G0 cells and increasing from G1 through mitosis.
Ki-67 antibodies are useful for assessing the growth fraction in neoplasms, with prognostic value comparable to thymidine-labelling index.
Commonly used as a neuronal marker of cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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