Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), FITC, eBioscience
Ki-67 단백질을 인식하는 20Raj1 클론의 FITC 결합 단일클론 항체로, 세포 증식 마커 분석에 적합. Flow cytometry, IHC, ICC 등 다양한 응용 가능. 인간 및 개 시료 반응. 488 nm/520 nm 형광 특성.
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Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | View 15 publications |
| ELISA | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 20Raj1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10686657 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450 / 506 / 570 / 615 / 660
- PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein, expressed in dividing cells (345 and 395 kDa isoforms). Ki-67 is detectable during active cell cycle phases (G1, S, G2, mitosis) and absent in resting cells (G0). During interphase, Ki-67 localizes to the nucleus and associates with heterochromatin-bound proteins such as CBX3 during mitosis. In tumor studies, Ki-67 serves as a marker for proliferating cell fraction.
This antibody also recognizes canine Ki-67.
Applications Reported: Intracellular staining for flow cytometry, immunohistochemistry, and immunocytochemistry.
Applications Tested: Pre-titrated and tested by intracellular staining and flow cytometry using Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Use at 5 µL (0.06 µg) per test (final volume 100 µL; 10⁵–10⁸ cells/test).
Excitation: 488 nm; Emission: 520 nm; Laser: Blue.
Filtered post-manufacturing (0.2 µm).
Target Information
Ki-67 is a nuclear protein expressed during the cell cycle (G1, S, G2, M phases) and absent in G0. It associates with euchromatin and heterochromatin regions and shows phase-specific localization. Ki-67 is widely used as a proliferation marker in tumor and neuronal studies, correlating with tumor grade and cell growth fraction.
For Research Use Only. Not for use in diagnostic procedures.
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