Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), FITC, eBioscience
Ki-67 단백질을 인식하는 SolA15 클론의 형광 표지 단일클론 항체로 세포 증식 분석에 사용됩니다. Flow cytometry, IHC, ICC 등 다양한 응용 가능. FITC 형광(Ex/Em 498/517 nm)으로 표지되어 있으며, Mouse, Rat, Human 등 다종 반응성.
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Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 |
| Immunohistochemistry (IHC) | Assay-Dependent | 70 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 3 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | 45 |
| Flow Cytometry (Flow) | 0.25 µg/test | 113 |
| Functional Assay | - | 1 |
| Miscellaneous PubMed | - | 1 |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat |
| Published Species | Guinea pig, Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SolA15 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151689 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700, Plus 594
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506, 570, 615, 660
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, and mitosis) but absent from resting cells (G0). Ki-67 redistributes from the nucleus to chromosomes during mitosis and serves as a marker for cell proliferation. The Ki-67 labeling index is used to predict outcomes in tumor studies.
SolA15 also recognizes human, non-human primate, and canine Ki-67.
Applications Reported: Intracellular staining for flow cytometry, immunohistochemistry, and immunocytochemistry.
Applications Tested:
- ICC on fixed OLN93 cells ≤10 µg/mL
- Flow cytometry on stimulated mouse splenocytes ≤0.25 µg/test using Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
A test is defined as staining a cell sample in 100 µL final volume (10⁵–10⁸ cells/test).
Careful titration is recommended for optimal performance.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Ki-67 is a nuclear protein expressed during cell cycle phases G1, S, G2, and M. It associates with euchromatin and heterochromatin through its forkhead-associated domain (FHA). Ki-67 expression varies by cell cycle stage and is absent in G0 cells, making it a reliable proliferation marker. In neoplastic tissues, the Ki-67 index correlates with tumor grade and prognosis, and it is routinely used as a marker of cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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