Thermo Fisher Scientific Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:250

View 19 publications 19 publications

Immunohistochemistry (IHC)

-

View 189 publications 189 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:125

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

1:50-1:125

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:125

View 30 publications 30 publications

Flow Cytometry (Flow)

-

View 3 publications 3 publications

Immunomicroscopy (IM)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Avian, Chicken, Goat, Human, Mouse, Non-human primate, Sheep, Pig, Rat, Xenopus

Published species

Bovine, Cat, Dog, Human, Lizard, Mouse, Non-human primate, Pig, Rabbit, Rat, Rhesus monkey, Sheep

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

1A4

Immunogen

Peptide corresponding to residues E(1) E E D S T A L V C(10) of alpha-smooth muscle actin with an acetylated n-terminus coupled to KLH. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Alpha-Smooth Muscle Actin,uniProtId:P62736-1,ncbiNodeId:9606,antigenRange:1-10,antigenLength:377,antigenImageFileName:MA1-06110_Alpha-Smooth_Muscle_Actin_P62736-1_House_mouse.svg,antigenImageFileNamePDP:MA1-06110_Alpha-Smooth_Muscle_Actin_P62736-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 420
• Alexa Fluor 514
• Alexa Fluor 700
• Alexa Fluor Plus 594
• Alexa Fluor Plus 750
• Request custom conjugation

Form

Liquid

Concentration

1.0 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_557419

Product Specific Information

MA1-06110 detects a-smooth muscle actin (a-SM1) in human, rat, goat, porcine, non-human primate, quail, ovine, xenopus, monkey and chicken samples. Since the epitope recognized by MA1-06110 is highly conserved, it can also cross-react with protochordates, lower craniates and mammals other than those specifically listed.

MA1-06110 has sucessfully been used in immunocytochemistry, immunomicroscopy, immunohistochemistry, and Western blotting procedures.

The MA1-06110 immunogen is a peptide corresponding to residues E(1) E E D S T A L V C(10) of alpha-smooth muscle actin with an acetylated n-terminus coupled to KLH. MA1-06110 specifically recognizes the epitope Ac-EEED on alpha-smooth muscle actin.

Target Information

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.