Thermo Fisher Scientific E-cadherin Monoclonal Antibody (MB2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:200

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

1:100-1:200

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

MB2

Immunogen

MCF-7/AZ cells expressing E-cadherin at their cell surface. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:E-cadherin,uniProtId:P12830-1,ncbiNodeId:9606,antigenRange:1-882,antigenLength:882,antigenImageFileName:MA1-06300_E-cadherin_P12830-1_House_mouse.svg,antigenImageFileNamePDP:MA1-06300_E-cadherin_P12830-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_557849

Product Specific Information

MA1-06300 detects E-cadherin in human samples.

MA1-06300 has sucessfully been used in Western blotting, Flow cytometry, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry. By Western blotting, it detects a ~120 kDa protein representing E-cadherin.

The MA1-06300 immunogen is MCF-7/AZ cells expressing E-cadherin at their cell surface.

Store at 4ºC, or in small aliquots at -20ºC.

Target Information

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.