Thermo Fisher Scientific CDH11 Monoclonal Antibody (16A)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA1-06306 detects OB-cadherin in human and rat samples.
It has been successfully used in Western blotting, immunocytochemistry, and immunohistochemistry.
The immunogen is the affinity purified extracellular domain of human cadherin-11-GST fusion protein.
Store at 4°C, or in small aliquots at -20°C.

Target Information

This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion.
All cadherins are single-pass transmembrane proteins with a variable number of 110 amino acid extracellular cadherin (EC) domains.
Classical cadherins contain five large N-terminal EC domains, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain.

Based on amino acid alignment, classical cadherins are divided into type I and type II subgroups.
Type II cadherins include human cadherin-5, -6, -8, -11, and -12, defined by their lack of a HAV cell adhesion recognition sequence specific to type I cadherins.

Cadherin-11 (OB-Cadherin) is a marker of the loosely connected and migratory cellular elements of the mesenchyme.
Strong expression has been noted in brain, spinal cord, bone marrow, and bone cells, and is regulated in endometrial glandular epithelial and stromal cells.
Its expression in osteoblastic cell lines and upregulation during differentiation suggest a role in bone development and maintenance.

A variant Cadherin-11 gene encodes a truncated protein with an unusual cytoplasmic domain generated by alternative splicing.
Cadherin-11 mRNA and protein are expressed in most invasive breast cancer cell lines but not in non-invasive lines, suggesting a potential role as a marker for aggressive tumor subsets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.