Thermo Fisher Scientific CDH11 Monoclonal Antibody (16A)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:25-1:250

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:50

-

Immunocytochemistry (ICC/IF)

1:10-1:50

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

16A

Immunogen

Affinity purified, extracellular domain of human cadherin-11-GST fusion protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2275953

Product Specific Information

MA1-06306 detects OB-cadherin in human and rat samples.

MA1-06306 has sucessfully been used in Western blotting, immunocytochemistry, and immunohistochemistry.

The MA1-06306 immunogen is affinity purified extracellular domain of human cadherin-11-GST fusion protein.

Store at 4ºC, or in small aliquots at -20ºC.

Target Information

This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. All cadherins are single-pass transmembrane proteins with a variable number of 110 amino acid extracellular cadherin (EC) domains. Classical cadherins contain five large N-terminal EC domains, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Based on amino acid alignment, classical cadherins are divided into type I and type II subgroups. Type I cadherins, including cadherins E (epithelial), N (neural), P (placental), and R (retinal) cadherin differ from type I cadherins in their specific amino acid sequences. Type II cadherins include human cadherin-5, -6, -8, -11, and -12. Type II cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Cadherin-11 (OB-Cadherin) is a marker of the loosely connected and migratory cellular elements of the mesenchyme. Strong expression of Cadherin-11 has been noted in brain, spinal cord, bone marrow and bone cells, and is regulated in human endometrial glandular epithelial and stromal cells. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. A variant Cadherin-11 gene (variant form) encodes a truncated protein with an unusual cytoplasmic domain not present in other cadherins. The variant protein is generated by alternative splicing, utilizing a 179 bp intronic sequence. Recently, Cadherin-11 mRNA and protein were found to be expressed in most invasive breast cancer cell lines tested but not in non-invasive cell lines, suggesting that Cadherin-11 may be a marker for aggressive, invasive tumor subsets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.