Thermo Fisher Scientific Cardiotin Monoclonal Antibody (SR-4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20-1:250

-

Immunohistochemistry (Frozen) (IHC (F))

1:25-1:50

-

Product Specifications

Species Reactivity

Human, Pig

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

SR-4

Immunogen

Cardiotin purified from mouse spleen cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_557933

Product Specific Information

MA1-06310 detects cardiotin in human and porcine samples.

MA1-06310 has sucessfully been used in Western blotting and immunohistochemistry. By Western blotting, it detects a ~300 kDa protein representing cardiotin from.

The MA1-06310 immunogen is cardiotin purified from mouse spleen cells.

Target Information

Cardiotin is a recently discovered, high molecular weight protein complex (~300 kDa) located in the longitudinal SR of cardiomyocytes. The cardiotin complex exists as 60 kDa and 100 kDa subunits. Both subunits contain the same amino-terminal 14 amino-acid sequence, and show high homology to human skeletal muscle alpha-actin. The tetrameric configuration of the cardiotin protein complex suggests that it exists as a transmembrane complex with the N-terminus at the cytoplasmic side of the membrane, able to interact with actin. Immunofluorescence studies colocalize cardiotin with SERCA 2a ATPase in the longitudinal sarcoplasmic reticulum (SR), although cardiotin is not expressed in junctional SR with SERCA 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.