Thermo Fisher Scientific Cardiotin Monoclonal Antibody (SR-4)

Thermo Fisher Scientific Cardiotin Monoclonal Antibody (SR-4)

Applications and Tested Dilution

• Western Blot (WB): 1:20–1:250
• Immunohistochemistry (Frozen) (IHC-F): 1:25–1:50

Product Specifications

Product Specific Information

MA1-06310 detects cardiotin in human and porcine samples.
It has successfully been used in Western blotting and immunohistochemistry.
By Western blotting, it detects a ~300 kDa protein representing cardiotin.
The immunogen is cardiotin purified from mouse spleen cells.

Target Information

Cardiotin is a high molecular weight protein complex (~300 kDa) located in the longitudinal sarcoplasmic reticulum (SR) of cardiomyocytes.
It consists of 60 kDa and 100 kDa subunits, both sharing the same amino-terminal 14 amino-acid sequence and showing high homology to human skeletal muscle alpha-actin.
The tetrameric configuration suggests a transmembrane complex with the N-terminus at the cytoplasmic side, interacting with actin.
Immunofluorescence studies show colocalization of cardiotin with SERCA 2a ATPase in the longitudinal SR, but not in junctional SR with SERCA 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.