Thermo Fisher Scientific PPAR alpha Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 33 publications 33 publications

Immunohistochemistry (IHC)

-

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 5 publications 5 publications

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Avian, Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) V D T E S P I C P L S P L E A D D(18) C of mouse PPAR alpha. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PPAR alpha,uniProtId:P23204-1,ncbiNodeId:10090,antigenRange:1-18,antigenLength:468,antigenImageFileName:PA1-822A_PPAR_alpha_P23204-1_Rabbit.svg,antigenImageFileNamePDP:PA1-822A_PPAR_alpha_P23204-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2165595

Product Specific Information

PA1-822A detects peroxisome proliferator activated receptor (PPAR) alpha from mouse samples.

PA1-822A has been successfully used in Western blot, immunoprecipitation and immunofluoresence procedures. By Western blot, this antibody detects an ~52 kDa protein representing PPAR alpha from 3T3-L1 cell lysate. Immunofluoresence staining is observed predominantly in the nucleus as well as the cytoplasm.

PA1-822A immunogen is a synthetic peptide corresponding to residues M(1) V D T E S P I C P L S P L E A D D(18) C of mouse PPAR alpha. A single amino acid substitution (I8 to L8) exists between the mouse and human protein. This immunizing peptide (Cat.# PEP-025) is available for use in neutralization and control experiments.

Target Information

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR`s can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.