Thermo Fisher Scientific c-Jun Polyclonal Antibody
Thermo Fisher Scientific의 c-Jun Polyclonal Antibody는 mouse c-Jun 단백질을 검출하는 rabbit IgG 항체로, Western blot 및 ChIP assay에 최적화되어 있습니다. 합성 펩타이드 면역원 기반으로 제작되었으며, -20°C에서 안정적으로 보관 가능합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 10 µg/mL | View 1 publication |
| ChIP assay (ChIP) | 1–3 µL | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse c-Jun |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2129865 |
Product Specific Information
PA1-833 detects c-Jun from mouse cells.
PA1-833 has been successfully used in Western blot and ChIP procedures.
By Western blot, this antibody detects an ~40 kDa protein representing c-Jun in NIH 3T3 serum-stimulated cells.
A smaller band is also detected at ~26 kDa, possibly representing a proteolytic fragment of c-Jun.
The PA1-833 immunogen is a synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse c-Jun.
Reconstitute in 200 µL PBS to create a stock of 1 mg/mL.
Target Information
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
It promotes activity of NR5A1 when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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