Thermo Fisher Scientific c-Jun Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

10 µg/mL

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ChIP assay (ChIP)

1-3 µL

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Product Specifications

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:c-Jun,uniProtId:P05627-1,ncbiNodeId:10090,antigenRange:92-105,antigenLength:334,antigenImageFileName:PA1-833_c-Jun_P05627-1_Rabbit.svg,antigenImageFileNamePDP:PA1-833_c-Jun_P05627-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2129865

Product Specific Information

PA1-833 detects cJun from mouse cells.

PA1-833 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ~40 kDa protein representing cJun in NIH 3T3 serum stimulated cells. A smaller band is also detected at ~26 kDa possibly representing a proteolytic fragment of cJun.

The PA1-833 immunogen is a synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun.

Reconstitute in 200 µL PBS to create a stock of 1 mg/mL.

Target Information

c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5-TGA\[CG\]TCA-3. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.