Thermo Fisher Scientific c-Fos Polyclonal Antibody
Thermo Fisher Scientific의 c-Fos Polyclonal Antibody는 인간 및 생쥐 시료에서 c-Fos 단백질을 검출하는 Rabbit IgG 항체입니다. Western blot과 면역형광에 적합하며, 합성 펩타이드 면역원 기반으로 높은 특이성과 재현성을 제공합니다. 연구용으로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:500 | View publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues S(4) G F N A D Y E A S S S R C(17) of human c-Fos |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2106777 |
Product Specific Information
PA1-830 detects c-Fos from mouse and human cells.
It has been successfully used in Western blot and immunofluorescence procedures.
By Western blot, this antibody detects an ~60 kDa protein representing c-Fos in NIH 3T3 serum-stimulated cells.
The immunogen sequence is completely conserved in mouse c-Fos.
Reconstitute with 200 µL of PBS (1 mg/mL).
Target Information
FBJ murine osteosarcoma viral oncogene homolog, commonly known as Cellular protooncogene Fos (c-Fos), belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14.
c-Fos heterodimerizes with c-Jun to form the Activator Protein-1 (AP-1) transcription factor, which regulates transcription of several genes involved in cellular signal transduction, proliferation, and differentiation.
c-Fos is phosphorylated by various stimuli, including growth factors and insulin, at multiple sites.
The ERK MAPK kinase phosphorylates c-Fos at Threonine 232, regulating its nuclear localization and transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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