Thermo Fisher Scientific Phospho-mTOR (Ser2448) Monoclonal Antibody (MRRBY), eFluor 660, eBioscience

Thermo Fisher Scientific Phospho-mTOR (Ser2448) Monoclonal Antibody (MRRBY), eFluor 660, eBioscience

Applications

• Immunohistochemistry (IHC)
  • Tested Dilution: -
  • View 1 publication
• Flow Cytometry (Flow)
  • Tested Dilution: 5 µL (0.5 µg)/test
  • View 4 publications

Product Specifications

Product Specific Information

This MRRBY monoclonal antibody recognizes human and mouse mammalian target of rapamycin (mTOR, FRAP, RAFT) phosphorylated on S2448.
mTOR is a serine/threonine kinase that regulates cell growth, proliferation, motility, survival, protein synthesis, and transcription.
It acts as an ATP and amino acid sensor, balancing nutrient availability with cellular metabolism.
Phosphorylation at S2448 occurs via the PI3K/Akt signaling pathway and is linked to tumorigenesis, making mTOR a key target in cancer research.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis.

Applications Tested:

• Pre-titrated for intracellular staining of human peripheral blood cells.
• Recommended usage: 5 µL (0.5 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).

Staining Protocols:

• Protocol A: Two-step for cytoplasmic proteins (flexible for surface and intracellular detection).
• Protocol B: One-step for nuclear proteins (ideal for transcription factors).
• Protocol C: Two-step Fixation/Methanol for phospho-specific signaling discrimination.
  Detailed protocols are available in the BestProtocols® Section.

Fluorochrome Information:

• eFluor® 660 replaces Alexa Fluor® 647.
• Emission: 659 nm; Excitation: 633 nm (Red Laser).
• Ensure instrument compatibility.
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FRAP1 (mTOR) is a serine/threonine kinase critical for cellular growth and proliferation.
Alterations in the mTOR/PI3K/AKT pathway are associated with various cancers.
FRAP1 is the target of rapamycin and its analogues, used in immunosuppression and cancer therapy.
Mutations in this gene cause Smith-Kingsmore syndrome.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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