Thermo Fisher Scientific Musashi-2 Monoclonal Antibody (C1), eFluor 660, eBioscience
Musashi-2 단백질을 인식하는 Mouse monoclonal antibody로, eFluor 660 형광 표지된 제품입니다. IHC 및 ICC/IF에 사용 가능하며, 줄기세포 및 종양세포 연구에 적합합니다. 651/668 nm 형광 특성을 가지며, 4°C 보관 및 동결 금지 조건입니다.
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Thermo Fisher Scientific Musashi-2 Monoclonal Antibody (C1), eFluor 660, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG3, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C1 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2574344 |
Product Specific Information
The monoclonal antibody C1 recognizes human and mouse Musashi-2 (Msi2), a highly conserved RNA-binding protein. Musashi-2 contains two RNA-recognition domains and is expressed as isoforms of 36 and 37 kDa. Musashi-2 and Musashi-1 are expressed at high levels in stem and tumor cells. During neural development, Musashi-2 is expressed in progenitor cells in the ventricular and subventricular zones, while postnatally, its expression is limited to subsets of neurons, astrocytes, and adult stem cell populations.
Functionally, Musashi-2 associates with Sox2 and is required for self-renewal of embryonic stem cells. It plays a similar role in hematopoietic stem cells and is overexpressed in human myeloid leukemia, correlating with decreased survival. Musashi-2 may serve as a prognostic marker for acute myeloid leukemia (AML).
Applications Reported:
- Immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections
- Microscopy
- Immunocytochemistry
Applications Tested:
Tested by IHC using high pH antigen retrieval; recommended ≤ 5 µg/mL with titration for optimal performance.
Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647, emits at 659 nm, and is excited with a red laser (633 nm). Ensure instrument compatibility.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Msi2 (musashi homolog 2), also known as MSI2H, is a 328 amino acid cytoplasmic protein containing two RRM (RNA recognition motif) domains. It is expressed ubiquitously at low levels and regulates target mRNA expression, contributing to stem cell proliferation and maintenance in the CNS. Msi2 is phosphorylated posttranslationally and upregulated after brain injury, suggesting a role in tissue regeneration. Chromosomal aberrations involving Msi2 are associated with chronic myeloid leukemia progression. Multiple isoforms exist due to alternative splicing.
Tissue Specificity: Ubiquitous; detected at low levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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