Thermo Fisher Scientific CD4 Monoclonal Antibody (CC30)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

1:100

-

Flow Cytometry (Flow)

1:25-1:200

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

CC30

Immunogen

Bovine thymocytes

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2745323

Product Specific Information

This antibody recognizes approximately a 50 kDa transmembrane molecule considered to be the bovine homologue of human CD4. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.