Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), Alexa Fluor 700, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532
• APC, APC-eFluor 780
• Biotin, eFluor 450, FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series (various wavelengths)
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright series (436–780)
• Custom conjugation available

Product Specific Information

The OKT8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimers or CD8αα homodimers. CD8a is found on thymocytes, mature T cells, and NK cells. It binds to MHC class I and interacts with protein tyrosine kinase p56lck, playing a key role in T-cell development and activation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of human peripheral blood leukocytes. Recommended usage ≤0.125 µg/test (100 µL volume). Cell number can range from 10⁵–10⁸ cells/test. Optimize titration for best results.

Alexa Fluor® 700 emits at 723 nm and is excited by the red laser (633 nm). Recommended optical setup: 685 LP mirror and 710/20 filter.
Excitation: 633–647 nm; Emission: 723 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of Differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family, essential for signal transduction and T-cell differentiation/activation. CD8 acts as a co-receptor with TCR for MHC-I molecules. It exists as CD8αβ heterodimers or CD8αα homodimers on T cells, NK cells, monocytes, and dendritic cells. Binding of MHC-I/peptide complexes triggers Lck recruitment, leading to lymphokine production and cytotoxic T lymphocyte activation. Activated CTLs are crucial for pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is promoted by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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