Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), Alexa Fluor 700, eBioscience
인간 CD8a 항원을 인식하는 OKT8 클론의 단클론 항체로, Alexa Fluor 700 형광 표지. Flow cytometry에 최적화되어 있으며 0.125 µg/test로 사용 가능. T 세포 및 NK 세포 표면 CD8a 검출에 적합하며 안정적인 형광 신호 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 26 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT8 (OKT-8) |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657756 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532
- APC, APC-eFluor 780
- Biotin, eFluor 450, FITC
- Functional Grade
- NovaFluor Blue, Red, Violet, Yellow series (various wavelengths)
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-eFluor 710
- Super Bright series (436–780)
- Custom conjugation available
Product Specific Information
The OKT8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimers or CD8αα homodimers. CD8a is found on thymocytes, mature T cells, and NK cells. It binds to MHC class I and interacts with protein tyrosine kinase p56lck, playing a key role in T-cell development and activation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of human peripheral blood leukocytes. Recommended usage ≤0.125 µg/test (100 µL volume). Cell number can range from 10⁵–10⁸ cells/test. Optimize titration for best results.
Alexa Fluor® 700 emits at 723 nm and is excited by the red laser (633 nm). Recommended optical setup: 685 LP mirror and 710/20 filter.
Excitation: 633–647 nm; Emission: 723 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Cluster of Differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family, essential for signal transduction and T-cell differentiation/activation. CD8 acts as a co-receptor with TCR for MHC-I molecules. It exists as CD8αβ heterodimers or CD8αα homodimers on T cells, NK cells, monocytes, and dendritic cells. Binding of MHC-I/peptide complexes triggers Lck recruitment, leading to lymphokine production and cytotoxic T lymphocyte activation. Activated CTLs are crucial for pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is promoted by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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