Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), Alexa Fluor 700, eBioscience
인간 CD4 항원에 특이적인 RPA-T4 단클론 항체로, Alexa Fluor 700 형광체로 표지되어 있습니다. Flow cytometry에 최적화되어 있으며 HIV 결합 차단 기능을 가집니다. 633 nm 레이저로 여기되고 723 nm에서 방출됩니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), Alexa Fluor 700, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 56 publications |
| Neutralization (Neu) | - | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RPA-T4 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation/Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11219880 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 496 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711
- eFluor 450 / 506
- FITC
- Functional Grade
- NovaFluor Blue 510 / 585 / 690
- Pacific Orange
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom conjugation available upon request
Product Specific Information
The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by thymocytes, mature T-helper cells, and low levels on monocytes. CD4 serves as a receptor for HIV, and RPA-T4 blocks HIV binding and mixed lymphocyte reaction.
RPA-T4 recognizes a different epitope than OKT4, and the antibodies do not cross-block each other.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.25 µg) per test.
A test is defined as the amount of antibody staining a cell sample in 100 µL final volume (10⁵–10⁸ cells/test).
Fluorochrome Details:
- Alexa Fluor® 700 emits at 723 nm and is excited with a 633 nm red laser.
- Suggested optics: 685 LP mirror and 710/20 filter.
- Excitation: 633–647 nm; Emission: 723 nm; Laser: Red laser.
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD4 antigen recognizes MHC class II molecules and acts as a co-receptor for HIV. It is expressed in T-helper lymphocytes, monocytes, macrophages, and dendritic cells.
Tissue expression includes thymus, lymph nodes, tonsils, spleen, and some brain and gut regions.
CD4 participates in early T-cell activation through association with the T-cell receptor complex and protein tyrosine kinase Lck.
It may also mediate neuronal damage in infectious and immune-mediated CNS diseases.
Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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