Thermo Fisher Scientific Ea52-68 peptide bound to I-Ab Monoclonal Antibody (eBioY-Ae (YAe, Y-Ae)), Biotin, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• Publications: View 15 publications

Product Specifications

Available Formats

• Unconjugated
• FITC
• Request custom conjugation

Product Specific Information

The Y-Ae antibody reacts with a class II major histocompatibility complex (MHC) self Ea peptide (peptide 52–68) bound to I-Ab molecules. It detects a determinant expressed on a subset of class II I-Ab molecules in strains that also express class II I-Eb. This determinant is found on peripheral B cells and thymic medulla cells, but not on thymic cortical epithelium.

The Y-Ae determinant is also expressed on macrophages and dendritic cells but does not react with invariant chain-associated class II MHC complexes. The antibody reactivity is MHC-restricted and was generated by immunization of [B10.MBR x B10.D2]F1 recipients with B10A(5R) LPS blasts.

It reacts with 10–15% of surface I-Ab molecules in strains expressing surface I-Eb molecules (e.g., B10.A(5R), B10.A(3R)), but not with I-A molecules in strains lacking functional I-E (e.g., B6, B10) or mutant I-A molecules (e.g., (B6.c-H-2bm-12 x A.TFR5)F1). The antibody recognizes Ea peptide (Ea 52–68) bound to I-Ab, but not to I-Ak or I-Abm-12 molecules.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse strains expressing I-A^b/MHC Class II:Ea peptide complex. Recommended ≤0.5 µg/test (100 µL final volume). Optimal concentration should be titrated per assay.

Target Information

MHC class II molecules are heterodimers composed of alpha (31–34 kDa) and beta (26–29 kDa) chains. Antigen presentation by MHC class II requires antigen degradation into peptides and removal of the invariant chain (Ii) to enable peptide binding. These molecules present exogenous antigens to CD4+ T helper cells, with CD4 interacting with non-polymorphic MHC class II residues.

HLA and MHC antibodies are critical tools in Immunopeptidomics, supporting identification and characterization of neoantigens via high-performance liquid chromatography and tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.