Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Biotin, eBioscience
마우스 MHC Class II (I-A/I-E) 인식 단클론 항체로 다양한 하플로타입의 B세포, 대식세포, 수지상세포 및 활성화된 T세포 분석에 사용됩니다. Flow cytometry, IHC, ICC/IF 등 다중 응용 가능하며, Biotin으로 표지되어 있습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 18 |
| Immunohistochemistry (PFA fixed) | - | 1 |
| Immunohistochemistry (Frozen) | - | 2 |
| Immunocytochemistry (ICC/IF) | - | 5 |
| Flow Cytometry (Flow) | 0.06 µg/test | 97 |
| ELISA | - | 1 |
| Immunoprecipitation (IP) | - | 2 |
| Miscellaneous PubMed | - | 3 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M5/114.15.2 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466661 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 700
- APC, APC-eFluor 780
- Brilliant UV 496, 661, 737
- Brilliant Violet 421, 605, 650, 711, 786
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue, Red, Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
The M5/114.15.2 monoclonal antibody reacts with mouse major histocompatibility complex (MHC) class II molecules, recognizing both I-A and I-E subregion-encoded glycoproteins (I-A^b, I-A^d, I-A^q, I-E^d, I-E^k, but not I-A^f, I-A^k, or I-A^s).
It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice with H-2^b, H-2^d, H-2^q, H-2^p, H-2^r, and H-2^u haplotypes, but not H-2^s or H-2^f.
The M5/114 antibody inhibits I-A–restricted T cell responses of H-2^b, H-2^d, H-2^q, and H-2^u haplotypes.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; ≤0.06 µg per test recommended. A test is defined as the amount of antibody used in a 100 µL staining volume. Optimize titration for best performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
MHC class II molecules are transmembrane glycoproteins expressed on professional antigen-presenting cells such as macrophages, dendritic cells, and B cells.
They bind endocytosed exogenous antigens and present them to T cells. The antigen-binding groove between the MHC class II alpha and beta chains is open at both ends and accommodates peptides 15–24 amino acids long.
HLA and MHC antibodies are crucial in immunopeptidomics for identifying and characterizing neoantigens via high-performance liquid chromatography coupled with tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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