Thermo Fisher Scientific CD133 (Prominin-1) Monoclonal Antibody (13A4), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• FITC
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The 13A4 monoclonal antibody recognizes mouse Prominin-1 (CD133, human orthologue AC133), a 115–120 kDa pentaspan transmembrane glycoprotein. Prominin-1 is expressed on primitive cells such as hematopoietic, neural, and endothelial stem cells, retina and retinoblastoma, and developing epithelium.
The 13A4 antibody does not cross-react with rat, human, chicken, or Drosophila antigens but has been reported to work in canine samples.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.125 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD133 (AC133) is a 97 kDa glycoprotein with five transmembrane domains, appearing as a 120 kDa band due to glycosylation. It is expressed on immature hematopoietic stem cells and various progenitor cells, including neural and embryonic stem cells. CD133 expression is associated with several cancers, including leukemias and brain tumors, and is also found at low levels in kidney, pancreas, placenta, and fetal liver.
Mutations in CD133 are linked to retinitis pigmentosa and Stargardt disease. Expression is controlled by multiple alternative promoters and transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.