Thermo Fisher Scientific CD133 (Prominin-1) Monoclonal Antibody (13A4), Biotin, eBioscience
Mouse CD133 (Prominin-1) 인식 단클론 항체로, Biotin 결합 형태입니다. Flow cytometry에 최적화되어 있으며, Dog 및 Mouse에 반응합니다. 0.5 mg/mL 농도의 액상 제품으로 4°C 보관, 동결 금지입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 4 publications |
| Immunohistochemistry (IHC) | - | View 15 publications |
| Immunocytochemistry (ICC/IF) | - | View 7 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 30 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 13A4 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466590 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- FITC
- PE
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
The 13A4 monoclonal antibody recognizes mouse Prominin-1 (CD133, human orthologue AC133), a 115–120 kDa pentaspan transmembrane glycoprotein. Prominin-1 is expressed on primitive cells such as hematopoietic, neural, and endothelial stem cells, retina and retinoblastoma, and developing epithelium.
The 13A4 antibody does not cross-react with rat, human, chicken, or Drosophila antigens but has been reported to work in canine samples.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.125 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD133 (AC133) is a 97 kDa glycoprotein with five transmembrane domains, appearing as a 120 kDa band due to glycosylation. It is expressed on immature hematopoietic stem cells and various progenitor cells, including neural and embryonic stem cells. CD133 expression is associated with several cancers, including leukemias and brain tumors, and is also found at low levels in kidney, pancreas, placenta, and fetal liver.
Mutations in CD133 are linked to retinitis pigmentosa and Stargardt disease. Expression is controlled by multiple alternative promoters and transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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