Thermo Fisher Scientific CD282 (TLR2) Monoclonal Antibody (6C2), FITC, eBioscience
마우스 TLR2(CD282)를 인식하는 단클론 항체로, FITC로 표지되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 1 µg/test 사용 권장. 선천면역 및 염증 반응 연구에 적합하며, 4°C 암소 보관 권장.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD282 (TLR2) Monoclonal Antibody (6C2), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 17 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6C2 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465439 |
Available Formats:
- Unconjugated
- Biotin
- eFluor 660
- Functional Grade
- PE
- Custom conjugation available
Product Specific Information
The 6C2 monoclonal antibody reacts with mouse Toll-like receptor 2 (TLR2). Mouse TLR2 is expressed by the myeloid lineage, including macrophages and dendritic cells in splenocytes and the RAW264.7 cell line.
TLR2 and TLR4 act as pattern recognition receptors and signaling molecules in response to bacterial lipoproteins, playing key roles in innate immunity and inflammation.
Applications Reported:
6C2 has been reported for use in flow cytometric analysis. For optimal staining, directly conjugated 6C2 (FITC, PE, or Biotin) is recommended in combination with FcR blocking using anti-mouse CD16/CD32 (clone 93, Product #14-0161).
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤1 µg per test (defined as staining a cell sample in 100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody should be titrated for optimal performance.
Optical Properties:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
TLR2 is a member of the Toll-like receptor family, essential for pathogen recognition and activation of innate immunity.
It acts through adaptor molecules such as MyD88 and TIRAP to activate kinases and transcription factors including PKCα/β and NF-κB.
TLR2 forms heterodimers with TLR1 or TLR6 to recognize bacterial and mycoplasma lipoproteins.
It is highly expressed in peripheral blood leukocytes and mediates responses to Gram-positive bacteria and yeast.
Expression is regulated by immune modifiers: LPS, GM-CSF, IL-1, and IL-10 upregulate TLR2, while IL-4, IFN-γ, and TNF downregulate expression in monocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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