Thermo Fisher Scientific TLE1 Monoclonal Antibody (ZM93), MonoMab

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Ready-to-use (150–200 µL)

Product Specifications

Product Specific Information

This product is diluted and ready-to-use.
A recommended positive control tissue is Synovial sarcoma, though other tissues may also be suitable.

The antibody is intended for professional laboratory use in detecting the corresponding protein in formalin-fixed, paraffin-embedded tissue using manual qualitative immunohistochemistry (IHC). It is used after primary tumor diagnosis by conventional histopathology.

TLE genes are human homologs of the Drosophila Groucho gene, a transcriptional repressor involved in neurogenesis, segmentation, and sex determination. Transducin-like enhancer protein 1 (TLE1) plays roles in hematopoiesis, neuronal, and epithelial differentiation. Positive nuclear staining with anti-TLE1 aids in differentiating synovial sarcoma from other soft tissue malignancies.

Pretreatment of deparaffinized tissue with heat-induced or enzymatic epitope retrieval is recommended.
IHC staining visualizes antigens through sequential application of:

1. Primary antibody
2. Secondary (link) antibody
3. Enzyme complex
4. Chromogenic substrate

Enzymatic activation produces a visible reaction product at the antigen site, interpreted under a light microscope.

A positive tissue control must accompany every staining procedure. This tissue should contain both positive and negative staining cells to verify staining accuracy.
External positive controls should be freshly prepared and processed identically to patient samples. Weakly positive tissues are ideal for detecting subtle reagent degradation.

Negative control tissue may be internal or external, depending on institutional guidelines. Non-staining components confirm absence of non-specific staining. If specific staining appears in negative controls, results are invalid.

Target Information

Transcriptional corepressor that interacts with multiple transcription factors.

• Inhibits NF-kappa-B regulated gene expression.
• Suppresses transcriptional activation mediated by FOXA2, CTNNB1, and TCF family members in Wnt signaling.
• Function modulated by association with AES.
• Acts as coactivator for ESRRG.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.