Thermo Fisher Scientific TCP1 Monoclonal Antibody (23c), APC
TCP1 단백질 검출용 Rat 모노클로날 항체로 APC 형광표지되어 있습니다. Western blot, ICC/IF, IP 등 다양한 응용에 적합하며 4°C 보관 조건에서 안정적입니다. 60kDa 및 92kDa 단백질을 검출하며 인간 HSP60에는 반응하지 않습니다.
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Thermo Fisher Scientific TCP1 Monoclonal Antibody (23c), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Host / Isotype | Rat / IgG2c |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 23c |
| Immunogen | Purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp1b gene expressed in E. coli. Detects the COOH group. |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- 1 µg/mL of MA5-45715 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
- Detects approximately 60 kDa and 92 kDa proteins.
- Addition of alanine (LDDA COOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences.
- Recognizes other proteins, notably the p102B COP subunit of Golgi coatomer.
- Does not react with human HSP60 protein.
Target Information
The T Complex Polypeptide 1 (TCP-1) is an approximately 60 kDa protein constitutively expressed in nearly all eukaryotic cells and upregulated during spermatogenesis. It is located in the cytosol as a subunit of a hetero-oligomeric chaperone involved in the folding of actin and tubulin.
Chaperonins recognize and stabilize polypeptide intermediates during folding, assembly, and disassembly. They share characteristics with Heat Shock Protein 70 (HSP70), including high abundance, induction by stress, and ATPase activity.
The chaperonin family includes mitochondrial HSP60, E. coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. TCP-1 shares ~40% identity with TF55 but minimal similarity to HSP60 and GroEL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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