Thermo Fisher Scientific HSF1 Monoclonal Antibody (4B4), PerCP

Applications and Tested Dilution

Product Specifications

Additional Formats

• APC
• FITC
• Request custom conjugation

Product Specific Information

1 µg/mL of MA5-45714 was sufficient for detection of HSF1 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody. Detects approximately 85 kDa (unstressed cell lysates) and approximately 95 kDa (heat-shocked cell lysates).

Target Information

All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs contain three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and located upstream of heat shock genes.

Two HSFs have been identified in human cells: HSF1 and HSF2, which bind to the same HSEs and share 38% sequence identity. HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and nucleus as a monomer lacking DNA binding activity. Upon stress, HSF1 becomes a nuclear-localized trimer that binds DNA, and phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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