Thermo Fisher Scientific TCP1 Monoclonal Antibody (23c), FITC

Applications and Tested Dilution

Product Specifications

Product Specific Information

• 1 µg/mL of MA5-45716 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
• Detects approximately 60 kDa and also approximately 92 kDa.
• The addition of an alanine (LDDA COOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences.
• This antibody recognizes other proteins, notably the p102B COP subunit of Golgi coatomer.
• It does not react with human HSP60 protein.

Target Information

The T Complex Polypeptide 1 (TCP-1) is an approximately 60 kDa protein constitutively expressed in almost all eukaryotic cells and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone involved in the folding of actin and tubulin.

Chaperonins recognize and stabilize polypeptide intermediates during folding, assembly, and disassembly, sharing many characteristics with Heat Shock Protein 70 (HSP70), including high abundance, induction by environmental stress, and ATPase activity.

The chaperonin family includes mitochondrial HSP60, E. coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP-1 sequence shows nearly 40% identity to TF55 but only minimal similarity to HSP60 and GroEL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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