Thermo Fisher Scientific TCP1 Monoclonal Antibody (23c), PerCP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Host/Isotype

Rat / IgG2c

Class

Monoclonal

Type

Antibody

Clone

23c

Immunogen

Purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp 1b gene expressed in E. coli. Detects the COOH group. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TCP1,uniProtId:P11983-1,ncbiNodeId:10090,antigenRange:556,antigenLength:556,antigenImageFileName:MA5-45717_TCP1_P11983-1_Rat.svg,antigenImageFileNamePDP:MA5-45717_TCP1_P11983-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

PerCP PerCP PerCP

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

1 µg/mL of MA5-45717 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.|Detects approximately 60kDa. Also detects approximately 92kDa. The addition of an alanine (LDDA COOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences. This antibody recognizes other proteins, most notably the p102B` COP subunit of Golgi coatomer. It does not react with human HSP60 protein.

Target Information

The T Complex Polypeptide 1 (TCP-1) is approximately 60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP-1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.