Thermo Fisher Scientific TCP1 Monoclonal Antibody (23c), PerCP
TCP1 단백질 검출용 Rat 모노클로날 항체로 PerCP 형광 표지. WB, ICC/IF, IP 등 다양한 응용 가능. 1 mg/mL 액상 형태로 4°C 보관. 60kDa 및 92kDa 단백질 검출에 적합하며 연구용으로만 사용.
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- pk
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Thermo Fisher Scientific TCP1 Monoclonal Antibody (23c), PerCP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rat / IgG2c |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 23c |
| Immunogen | Purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp1b gene expressed in E. coli. Detects the COOH group. |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- 1 µg/mL of MA5-45717 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
- Detects approximately 60kDa and 92kDa.
- The addition of an alanine (LDDA COOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences.
- Recognizes other proteins, notably the p102B COP subunit of Golgi coatomer.
- Does not react with human HSP60 protein.
Target Information
The T Complex Polypeptide 1 (TCP-1) is an approximately 60 kDa protein constitutively expressed in almost all eukaryotic cells and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone involved in the folding of actin and tubulin.
Chaperonins recognize and stabilize polypeptide intermediates during folding, assembly, and disassembly. They share characteristics with Heat Shock Protein 70 (HSP70), including high abundance, induction by environmental stress, and ATPase activity.
The chaperonin family includes mitochondrial HSP60, E. coli GroEL, plastid Rubisco-subunit binding protein, and archaebacterial TF55. TCP-1 shows ~40% identity to TF55 but minimal similarity to HSP60 and GroEL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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