Thermo Fisher Scientific HSF1 Monoclonal Antibody (4B4), FITC
HSF1 단백질 검출용 FITC 결합 Rat 모노클로날 항체로, 다양한 종에 반응하며 WB, IHC, ICC, ELISA 등 다중 응용 가능. 고순도 Protein G 정제, 4°C 암소 보관. 연구용으로 열충격 반응 관련 단백질 분석에 적합.
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Thermo Fisher Scientific HSF1 Monoclonal Antibody (4B4), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Gel Shift (GS) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Guinea pig, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat |
| Host/Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4B4 |
| Immunogen | Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425–439 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932167 |
Product Specific Information
1 µg/mL of MA5-45713 was sufficient for detection of HSF1 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.
Detects approximately 85 kDa (unstressed cell lysates) and approximately 95 kDa (heat-shocked cell lysates).
Target Information
All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by heat shock factor (HSF), which binds to heat shock response elements (HSEs).
HSEs are three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation upstream of heat shock genes.
Two HSFs are identified in human cells: HSF1 and HSF2, sharing 38% sequence identity. HSF1 responds to classical stress signals (heat, heavy metals, oxidative reagents), while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA-binding activity. Under stress, HSF1 becomes activated into a nuclear-localized trimeric state that binds DNA. Phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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