Thermo Fisher Scientific CD40 Monoclonal Antibody (5C3), NovaFluor Red 685, eBioscience

Thermo Fisher Scientific CD40 Monoclonal Antibody (5C3), NovaFluor Red 685, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 690
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The 5C3 monoclonal antibody reacts with human CD40, a 45–50 kDa type I transmembrane glycoprotein.
CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells.
It regulates B cell development and maturation by inducing Ig isotype-switching and, with IL-4, protects B cells from apoptosis and promotes proliferation.

Interaction of CD40 with CD154 (gp39), its ligand on T cells, is critical for T-B cell crosstalk and immune regulation.
5C3 is used for activation of B cells in in vitro functional assays.

Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells (5 µL per test).

A test is defined as the amount of antibody staining a cell sample (100 µL final volume).
Cell number can range from 10⁵ to 10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes such as PI, 7-AAD, or DAPI.
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample.

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures with minimized spillover and spread.

Excitation: 637 nm
Emission: 685 nm
Laser: 633–640 nm (Red)

Target Information

CD40 is a single-chain glycoprotein, member of the TNFR/NGFR superfamily, and homologous to CD30.
It is expressed on all B cells except plasma cells, as well as epithelial cells, carcinomas, and lymphoid dendritic cells.
CD40 mediates immune and inflammatory responses, including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.

AKNA transcription factor regulates CD40 expression, important for cell interactions.
TNFR2 adaptor protein interacts with CD40 for signal transduction.
CD40-CD154 interaction is necessary for amyloid-beta-induced microglial activation, relevant to Alzheimer’s disease pathogenesis.

Two alternatively spliced transcript variants encode distinct CD40 isoforms.
Diseases associated with CD40 dysfunction include Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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