Thermo Fisher Scientific PARP1 (Cleaved Asp214) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PARP1(Accession P09874), corresponding to amino acid residues P200-K222. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PARP1 (cleaved Asp214),uniProtId:P09874-1,ncbiNodeId:9606,antigenRange:200-222,antigenLength:1014,antigenImageFileName:PA5-114686_PARP1_cleaved_Asp214_P09874-1_Rabbit.svg,antigenImageFileNamePDP:PA5-114686_PARP1_cleaved_Asp214_P09874-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2899322

Product Specific Information

Antibody detects endogenous levels of fragment of activated PARP resulting from cleavage adjacent to Asp214.

Target Information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.