Thermo Fisher Scientific Caspase 9 (Cleaved Asp315) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody detects endogenous levels of the fragment of activated Caspase 9 resulting from cleavage adjacent to Asp315.

Target Information

Caspase 9 (also known as ICE-like apoptotic protease 6, ICE LAP6, Mch6, Apaf3) is a member of the peptidase family C14 containing a CARD domain. Caspases are cysteine proteases expressed as inactive precursors that mediate apoptosis through proteolysis of specific substrates.
Caspase 9 functions as a heterotetramer, localizing in the cytosol and translocating to mitochondria upon activation. It participates in the caspase activation cascade responsible for apoptosis execution and activates Caspase 3 and Caspase 6.
Binding of Caspase 9 to Apaf-1 leads to activation of the protease, which then cleaves and activates Caspase 3. Caspase 9 promotes DNA damage-induced apoptosis in an ABL1/c-Abl-dependent manner and cleaves poly(ADP-ribose) polymerase (PARP).
Caspase 9 is expressed in various human tissues, and mutations can affect brain and neuron development. Dysregulated expression is associated with cancer and neurodegenerative diseases such as Alzheimer’s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.