Thermo Fisher Scientific Estrogen Receptor alpha Polyclonal Antibody, FITC
FITC로 표지된 에스트로겐 수용체 알파 폴리클로날 항체로, 인간 및 랫트 시료에 반응합니다. Western blot, ELISA, IP에 사용 가능하며 고순도 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용되며, 형광 검출에 최적화된 항체입니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
ELISA
- Tested Dilution: 1:10,000
Immunoprecipitation (IP)
- Tested Dilution: 1:50–1:250
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 520–570 on human Estrogen receptor alpha protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor acts as a ligand-activated transcription factor that, upon binding to estrogen, undergoes a conformational change enabling dimerization and interaction with estrogen response elements (ERE) in DNA. This binding regulates gene transcription by recruiting coactivator or corepressor proteins.
There are two forms of estrogen receptors, alpha (ESR1) and beta (ESR2), encoded by separate genes. At least four isoforms of estrogen receptor-alpha are known (Isoform 1: 66 kDa, Isoform 2: 53 kDa, Isoform 3: 47 kDa, Isoform 4: 35 kDa). These receptors are widely expressed across various tissues and are essential for sexual development, reproductive function, bone metabolism, and are implicated in pathological processes such as breast cancer, endometrial cancer, and osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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