Thermo Fisher Scientific Phospho-AKT (Thr308) Polyclonal Antibody
Phospho-AKT (Thr308) 폴리클로날 항체로 인간, 마우스, 랫트에 반응합니다. Western blot, IHC, ELISA 등 다양한 응용에 적합하며, 인산화된 AKT1 단백질을 특이적으로 인식합니다. 액상 형태로 제공되며 -20°C 보관을 권장합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Phospho-AKT (Thr308) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (IHC) | 1:200 |
| ELISA | 1:15,000 |
| Immunoprecipitation (IP) | 1:1,000 |
| Dot Blot (DB) | 5 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-AKT pT308 polyclonal antibody produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to residues surrounding threonine 308 of human AKT1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
This antibody is specific for phosphorylated human AKT; minimal reactivity occurs against non-phosphorylated AKT.
Reactivity against AKT from other species may occur but has not yet been tested.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival.
The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells.
This kinase is activated by insulin, PI3K, IGF1, and various other growth and survival factors.
Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9.
The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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