Thermo Fisher Scientific Phospho-IKK E (Thr501) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:2,000

-

ELISA (ELISA)

1:5,000-1:25,000

-

Immunoprecipitation (IP)

1:500-1:3,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IKKe phospho peptide corresponding to a region of the human protein surrounding pT501 conjugated to KLH. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IKK epsilon,uniProtId:Q14164-1,ncbiNodeId:9606,antigenRange:501,antigenLength:716,antigenImageFileName:600-401-267_IKK_epsilon_Q14164-1_Rabbit.svg,antigenImageFileNamePDP:600-401-267_IKK_epsilon_Q14164-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This phospho specific polyclonal antibody is specific for phosphorylated pT501 human IKKe. Reactivity with non-phosphorylated IKKe is minimal. Cross reactivity with pT501 phosphorylated IKKe from mouse, rat or other species has not been determined.

Target Information

IKBKE is an activator of nuclear factor-kappa-B. Over expression of IKBKE has been observed in breast cancer cells, and suppression of IKBKE induces cell death. NF-kappa-B is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappa-B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappa-B is associated with Ikappa-B proteins in the cell cytoplasm, which inhibit NF-kappa-B activity. Ikappa-B is phosphorylated by Ikappa-B kinase (IKK) complex that contains IKK-a, IKK-b, and IKK-gamma. A novel molecule in the IKK complex was recently identified and designated IKK-iota/IKK-i. IKK epsilon is required for the activation of NF-kappa-B by PMA and T cell receptors but not by TNF-a and IL-1. IKK-iota/IKK-i message is expressed in a variety of tissues and is inducible by TNF-a, IL-1, and LPS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.