Thermo Fisher Scientific Phospho-AKT (Ser473) Polyclonal Antibody
Phospho-AKT (Ser473) Polyclonal Antibody는 인간, 생쥐, 랫드에서 반응하며 AKT 단백질의 Ser473 인산화 형태를 특이적으로 인식합니다. Western blot, IHC, ICC/IF, ELISA 등 다양한 실험에 사용 가능하며, 고순도의 친화 크로마토그래피 정제 제품입니다. 연구용으로만 사용됩니다.
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- 600-401-268x (2개 옵션)
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:15,000–1:60,000 |
| Immunoprecipitation (IP) | 1:200–1:1,000 |
| Dot Blot (DB) | 1:70,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a C-terminus region near phospho Serine 473 of human, mouse, rat, and chicken AKT proteins conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
This antibody is specific for phosphorylated human AKTpS473. Minimal reactivity occurs against non-phosphorylated AKT.
Reactivity against AKT from other species may occur but has not yet been tested.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival.
The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells.
This protein kinase is activated by insulin, PI3K, IGF1, and various other growth and survival factors.
Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9.
The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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