Thermo Fisher Scientific Phospho-CREB (Ser133) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Store vial at –20°C prior to opening. Aliquot contents and freeze at –20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

This phospho-specific polyclonal antibody is specific for phosphorylated pS133 of human CREB. Reactivity with non-phosphorylated CREB is minimal. This antibody also shows cross-reactivity with pS133 phosphorylated CREB from mouse and rat.

Target Information

CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to extracellular signals such as neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB depends on phosphorylation of serine 133, mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase, and MSK1.

Although CREB binds DNA independent of its phosphorylation state, only the phosphorylated form functions as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator, binds to CREB in the region of serine 133. CREB is involved in processes such as circadian rhythm synchronization, adipocyte differentiation, and learning and memory. In humans, the CREB gene is located on the q arm of chromosome 2. Dysregulation of CREB has been associated with diseases including Alzheimer’s disease, histiocytoma, and soft tissue melanoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.