Thermo Fisher Scientific EpCAM (CD326) Monoclonal Antibody (323/A3), PE
EpCAM (CD326) 단일클론 항체(323/A3), PE 형은 인간 유래 세포를 대상으로 하는 Flow Cytometry용 항체입니다. EpCAM은 상피세포 및 다양한 암종에서 과발현되는 표지자로, PE 형광표지로 565/576 nm에서 검출됩니다. 연구용으로만 사용됩니다.
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Applications
- Flow Cytometry (Flow): 10 µL/10^6 cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 323/A3 |
| Immunogen | Human breast cancer MCF-7 cells |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898629 |
Additional Formats
- Unconjugated (MA1-10196)
- Alexa Fluor 488 (MA5-38713)
- Alexa Fluor 647 (MA5-38714)
- APC (MA5-38715)
- FITC (MA5-38716)
- PE-Cyanine7 (MA5-38718)
- PerCP-Cyanine5.5 (MA5-38719)
- Custom conjugation available
Product Specific Information
This antibody recognizes an extracellular epitope of CD326 / EpCAM, a marker of epithelial lineages that is over-expressed in many carcinomas.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism through induction of proto-oncogene c-myc and cell cycle regulators cyclin A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM is linked to enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors.
Ep-CAM is located on the basolateral surface of most simple epithelia and many carcinoma types and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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