Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (323/A3), FITC
EpCAM(CD326)을 인식하는 마우스 단클론 항체로, FITC로 표지되어 유세포분석에 적합. 인간 시료 반응성, 액상 형태로 제공되며 4°C 암소 보관. 다양한 형광 표지 형식으로도 제공 가능.
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Applications and Tested Dilution
- Flow Cytometry (Flow): 4 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 323/A3 |
| Immunogen | Human breast cancer MCF-7 cells |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898628 |
Available Formats
- Unconjugated (MA1-10196)
- Alexa Fluor 488 (MA5-38713)
- Alexa Fluor 647 (MA5-38714)
- APC (MA5-38715)
- PE (MA5-38717)
- PE-Cyanine7 (MA5-38718)
- PerCP-Cyanine5.5 (MA5-38719)
- Custom conjugation available upon request
Product Specific Information
This antibody recognizes an extracellular epitope of CD326 (EpCAM), a marker of epithelial lineages that is overexpressed in many carcinomas.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a Ca²⁺-independent homotypic cell adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cyclins A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is associated with enhanced epithelial proliferation and is a characteristic marker of epithelial-derived tumors.
Ep-CAM is expressed on the basolateral surface of most simple epithelia and many carcinoma types and can help distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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