Thermo Fisher Scientific EpCAM (CD326) Monoclonal Antibody (323/A3), Alexa Fluor 647
EpCAM(CD326)을 인식하는 마우스 단클론 항체로, Alexa Fluor 647로 표지되어 있습니다. 인간 시료에 반응하며 Flow Cytometry, ICC/IF, WB에 사용 가능합니다. 형광 최대치는 650/671 nm이며, 4°C 암소 보관이 필요합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 4 µL / 10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 323/A3 |
| Immunogen | Human breast cancer MCF-7 cells |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898626 |
Additional Formats
- Unconjugated (MA1-10196)
- Alexa Fluor 488 (MA5-38713)
- APC (MA5-38715)
- FITC (MA5-38716)
- PE (MA5-38717)
- PE-Cyanine7 (MA5-38718)
- PerCP-Cyanine5.5 (MA5-38719)
- Request custom conjugation available
Product Specific Information
This antibody recognizes an extracellular epitope of CD326 / EpCAM, a marker of epithelial lineages that is over-expressed in many carcinomas.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent cell adhesion molecule influencing cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cyclins A and E. Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, impacting epithelial cell differentiation and growth. Overexpression of Ep-CAM is associated with enhanced epithelial proliferation and is a marker for tumors of epithelial origin. It is expressed on the basolateral surface of most simple epithelia and many carcinoma types and can help distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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