Thermo Fisher Scientific TCL1 Monoclonal Antibody (eBio1-21 (1-21)), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 2 publications

Product Specifications

Available Formats

• Unconjugated
• eFluor 450
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The eBio1-21 antibody reacts with human T cell leukemia/lymphoma 1 (TCL1), a 14 kDa proto-oncogene product involved in intracellular regulation of T cell signaling. TCL1 was identified as the oncogene at the 14q32.1 chromosome breakpoint region in T-cell prolymphocytic leukemia (T-PLL). In T-PLL, TCL1 is overexpressed due to inversion or translocation near T-cell receptor promoter/enhancer elements.

TCL1 binds to the pleckstrin homology domain of Akt (protein kinase B) family proteins, facilitating Akt dimerization and activity. By increasing Akt activity, TCL1 enhances phosphorylation of downstream substrates such as Ikk complex, mTOR, BAD, p70S6 kinase, FOXO transcription factors, and GSK3β, which regulate differentiation, growth, survival, and metabolism.

TCL1 is normally expressed in CD3⁻CD4⁻CD8⁻ thymic precursors, plasmacytoid dendritic cells, stimulated mature T cells, and B cells up to the germinal center stage. Aberrant expression is associated with pre-malignant T cell expansions and mature T cell tumors. TCL1 expression is regulated in chronic lymphocytic leukemia (CLL).

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for human cell lines at 5 µL (0.25 µg)/test
• Recommended Cell Count: 10⁵–10⁸ cells/test
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Overexpression of the TCL1 gene in humans is implicated in mature T cell leukemia, where chromosomal rearrangements bring TCL1 near TCR-α or TCR-β regulatory elements (Virgilio et al., 1998). In normal T cells, TCL1 is expressed in CD4⁻/CD8⁻ cells but not in later differentiation stages. TCL1 functions as a coactivator of the cell survival kinase AKT (Laine et al., 2000).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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