Thermo Fisher Scientific CD97 Monoclonal Antibody (VIM3b), APC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

VIM3b

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10548515

Product Specific Information

Description: This VIM3b monoclonal antibody reacts with human CD97, a member of the epidermal growth factor (EGF)-TM7 family of cell surface receptors. Present on a variety of hematopoietic cells, CD97 is most highly expressed on granulocytes and monocytes. CD97 can also be detected on lymphocytes, with T and NK cells expressing higher levels than B cells. Moreover, CD97 expression on T lymphocytes can be induced by stimulation of CD3 and CD28. Studies have demonstrated that CD97 is involved in granulocyte trafficking and T cell activation. CD55, also known as Decay-accelerating factor (DAF), is the ligand of CD97.

Due to alternative splicing, CD97 exists as three isoforms with molecular weights of 74, 80, and 86 kDa. The VIM3b monoclonal antibody recognizes each form.

Applications Reported: This VIM3b antibody has been reported for use in flow cytometric analysis.

Applications Tested: This VIM3b antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.